Assess matrix effects and matrix-matched calibrationsSource:
Calculate the matrix effect by comparing the slope of a solvent-based calibration curve with one or more matrix-matched calibration. The matrix effect is expressed as signal suppression/enhancement ratio.
an object of class '
calibration' obtained from analyzing standard solutions of different concentration (solvent calibration data).
additional objects of the same type obtained from matrix-matched calibration data.
The magnitude of a matrix effect is estimated by subtracting the slope of a matrix-matched calibration from that of the solvent-based calibration. The difference is divided by the slope of the solvent-based calibration.
Matrix effects or signal suppression/enhancement ratios should be evaluated during analytical method development to avoid over- or underestimation of sample concentrations. In addition, signal suppression/enhancement ratios may help to justify the validity of a regular solvent calibration as opposed to matrix-matched calibrations. This may be the case if matrix effects or signal suppression/enhancement ratios are close to measurement repeatability.